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Analytical validation of a novel high multiplexing real-time PCR array for the identification of key pathogens causative of bacterial ventilator-associated pneumonia and their associated resistance genes.

par Bogaerts, P;Hamels, Sandrine;De Mendonça, Ricardo ;Huang, T D;Roisin, Sandrine ;Remacle, Josè;Markine-Goriaynoff, N;de Longueville, Francoise;Plüster, W;Denis, Olivier ;Glupczynski, Youri
Référence Journal of antimicrobial chemotherapy, 68, 2, page (340-347)
Publication Publié, 2013-02

Article révisé par les pairs

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Résumé :

Rapid diagnosis and appropriate empirical antimicrobial therapy before the availability of conventional microbiological results is of pivotal importance for the clinical outcome of ventilator-associated pneumonia (VAP). We evaluated the VAPChip, a novel, closed cartridge molecular tool aiming to identify directly from clinical samples and within a working day the principal bacteria causative of VAP as well as clinically relevant β-lactam resistance genes.