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Electron Microscope Observations on Virus Particles Associated With a Transplantable Renal Adenocarcinoma in BALB/cf/Cd Mice

par Claude, Albert ;Mrena, Emil ;Felluga, Bruno
Référence Journal of the National Cancer Institute, 43, 2, page (319-333)
Publication Publié, 1969

Article révisé par les pairs

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Résumé :

Andervont established the BALB/cf strain of mice as a high mammary tumor strain by foster nursing original young BALB/c on C3H mothers. The occurrence of endemic adenocarcinoma in this strain was first reported from this laboratory in 1958. Since then, a substrain (BALB/cf/Cd) has been developed by inbreeding and selection, with a renal tumor incidence of 60–70%. The spontaneous tumors are transplantable and a number of renal tumor cell lines have been maintained by grafting. The present work is an electron microscope study of one of these cell lines, the C+T5, maintained through more than 200 consecutive grafting generations, particularly from the point of view of virus particles (VP) which have been found associated with the tumor cells. From their morphology, morphogenesis, and distribution in and outside the cell, these VP resemble virus particle types observed in mammary tumors and lymphomas of mice. They can be carried over in cells through the process of mitosis. The BALB/cf inbred substrain, which in our stock has retained the original high mammary tumor incidence, is free of renal tumors, whereas the new BALB/cf/Cd high renal-tumor strain can no longer produce mammary tumors. The VP described in this paper are quite different, in their morphology, localization, and morphogenesis, from the VP previously described in another transplantable renal tumor cell line of the original BALB/c strain.