Article révisé par les pairs
| Résumé : | The pancreatic B-cell should be considered as a fuel sensor organ, its secretory response to nutrient secretagogues reflecting their capacity to augment catabolic fluxes. Hence, equal attention should be paid to the oxidation of exogenous and endogenous nutrients in islet cells. The identify of endogenous nutrients and their contribution to basal O2 uptake were recently examined. In the present study, the fate of the C atoms derived from endogenous nutrients was defined. Endogenous triglycerides, amino acids and glycolytic intermediates accounted, respectively, for 72.6%, 27.6% and 0.6% of total CO2 output. The islets also released pyruvate and lactate derived from endogenous amino acids (82.3%), glycerol liberated by hydrolysis of triglycerides (12.0%) and glycolytic intermediates (5.6%). This quantitative information allows computation of the basal generation rate of reducing equivalents in distinct metabolic pathways. |
