par Ingels, Aude 
;Rigutto, Sabrina ;Spruyt, Delphine ;Gangji, Valérie ;Mathieu, Myrielle ; [et al.]
Référence Association Research Circulation Osseous (ARCO) (Bruxelles, Belgium)
Publication Non publié, 2012-01
;Rigutto, Sabrina ;Spruyt, Delphine ;Gangji, Valérie ;Mathieu, Myrielle ; [et al.]Référence Association Research Circulation Osseous (ARCO) (Bruxelles, Belgium)
Publication Non publié, 2012-01
Poster de conférence
| Résumé : | Background: Non-union (NU) is defined as the absence of bone union 6 months after fracture. The prevalence of non- unions after a long bone fracture is around 3%. NU physiopathology is largely unknown but is usually seen as a vascular disease.Methods: NU patients (n=24) and healthy volunteers (HV, n=133) were included in this study. The number, the morphology, the phenotype, the auto/paracrine expression pattern and the biological activity of bone marrow-derived MSC were compared between groups. Serum level of growth factors and chemokines were assessed. Results: In NU compared to HV, we showed a lower number of osteoprogenitors cells by counting CFU-F (45,65 + 27,12 in HV vs 22,84 + 22,63 in NU, p=0,014) and measuring cell expansion in primary culture (13% + 9% for HV vs 5% + 3% for in NU, p < 0,001). However, once differentiated, osteoblasts from both groups present the same ability to mineralize. In accordance with these cellular results, we found abnormal level of growth factors in NU.Conclusion: This study shows abnormalities in osteoprogenitor cell function and differentiation, as well as impaired chemokines levels. Therefore, NU should also be considered as disease of the osteoblasts. |
