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Variations in cyclic AMP levels in the epididymal adipose tissue of normal and congenitally obese mice

par Dehaye, Jean-Paul ;Winand, Jacques ;Robberecht, Patrick ;Christophe, Jean
Référence Archives internationales de physiologie et de biochimie, 83, 1, page (177-178)
Publication Publié, 1975-02

Article révisé par les pairs

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Résumé :

In normal adipose tissue 2.5 x 10-6 M isoproterenol produced a 15 to 20 fold increase in cyclic adenosine monophosphate (cAMP) with a peak value of 250 pmoles/100 mg fresh tissue at 10 min. The cAMP levels decreased at 20 min and fell to control levels (12 pmoles/100 mg fresh tissue) after 1 hr. Half maximal stimulation of cAMP production was provoked by 1.5 x 10-7 M isoproterenol. The adipose tissue of obese mice responded poorly to isoproterenol stimulation. The maximal cAMP value (25 pmoles/100 mg fresh tissue) was only slightly higher than the control level (8 pmoles/100 mg fresh tissue) and to obtain this effect, a concentration of isoproterenol as high as 10-6 M was necessary. The adipose tissue of ob/ob mice aged 4 wk i.e., with no apparent signs of obesity, gave a similar response. Similar results were seen with synthetic corticotropin 1-24 10-6 M, i.e., the active peptide of adrenocorticotropic hormone, as lipolytic agent. Glucagon 10-5 M, vasoactive intestinal polypeptide 10-6 M and secretin 5 x 10-7 M were unable to increase cAMP levels, neither in the adipose tissue of normal mice nor in that of obese animals. However, adipose tissue of rats is sensitive to these three hormones, even at much lower concentrations.