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Endophytic fungi from leaves of Centella asiatica: occurrence and potential interactions within leaves

par Rakotoniriana, E.F;Munaut, C;Decock, Cony;Randriamampionona, Denis;Andriambololoniaina, M.;Rakotomalala, T.;Rakotonirina, El-C-Julio ;Rabemanantsoa, Christian;Cheuk, K.;Ratsimamanga, S.U;Mahillon, Jacques;El Jaziri, Mondher ;Quetin-Leclercq, Joëlle;Corbisier, Anne-Marie
Référence Antonie van Leeuwenhoek, 93, 1-2, page (27-36)
Publication Publié, 2008

Article révisé par les pairs

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Résumé :

Fungal endophytes were isolated from leaves of Centella asiatica (Apiaceae) collected at Mangoro (middle eastern region of Madagascar, 200 km from Antananarivo). Forty- five different taxa were recovered. The overall foliar colonization rate was 78%. The most common endophytes were the non-sporulating species 1 (isolation frequency IF 19.2%) followed by Colletotrichum sp.1 (IF 13.2%), Guignardia sp. (IF 8.5%), Glomerella sp. (IF 7.7%), an unidentified ascomycete (IF 7.2%), the non-sporulating species 2 (IF 3.7%) and Phialophora sp. (IF 3.5%). Using sequences of the ribosomal DNA internal transcribed spacer (ITS) regions, major endophytes (IF > 7%) were identified as xylariaceous taxa or as Colletotrichum higginsianum, Guignardia mangiferae and Glomerella cingulata. Results from in vitro fungal disk experiments showed a strong inhibitory activity of the xylariaceous non-sporulating species 1 against G. mangiferae and C. higginsianum and of C. higginsianum against G. mangiferae. This can be explained by antagonism between dominant taxa. © Springer Science+Business Media B.V. 2007.