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Single-step antigen loading and activation of dendritic cells by mRNA electroporation for the purpose of therapeutic vaccination in melanoma patients.

par Bonehill, Aude;Van Nuffel, An M T;Corthals, Jurgen;Tuyaerts, Sandra;Heirman, Carlo;Francois, Violaine ;Colau, Didier;van der Bruggen, P;Neyns, Bart;Thielemans, Kris M.
Référence Clinical cancer research, 15, 10, page (3366-3375)
Publication Publié, 2009-05

Article révisé par les pairs

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Résumé :

A critical factor determining the effectiveness of currently used dendritic cell (DC)-based vaccines is the DC activation or maturation status. We have recently shown that the T-cell stimulatory capacity of DCs pulsed with tumor-antigen-derived peptides can be considerably increased by activating the DCs through electroporation with mRNA encoding CD40 ligand, CD70, and a constitutively active Toll-like receptor 4 (TriMix DCs). Here, we investigate whether TriMix DCs can be coelectroporated with whole tumor-antigen-encoding mRNA.