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In-vitro maturation of human oocytes: before or after vitrification?

par Fasano, Giovanna ;Demeestere, Isabelle ;Englert, Yvon
Référence Journal of assisted reproduction and genetics, 29, 6, page (507-512)
Publication Publié, 2012-04

Article révisé par les pairs

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Résumé :

PURPOSE: This study aims to determine if in-vitro maturation (IVM) of human immature oocytes should be performed before or after vitrification. METHODS: A total of 184 immature oocytes were randomly divided into two different groups: 100 were vitrified at metaphase II (MII) stage 24 h-48 h after IVM (group 1) and 84 were immediately vitrified at germinal vesicle (GV) or metaphase I (MI) stages and in vitro matured after warming (group 2). RESULTS: Survival rate after warming was similar in both groups (86.9% versus 84.5%). However, oocyte maturation rate per collected oocyte was significantly higher for oocytes matured before vitrification (group 1, 46%) than for oocytes vitrified before IVM (group 2, 23.8%) (p < 0.01). Consequently, the number of MII oocytes inseminated per oocyte collected was significantly higher for group 1 (40%) than for group 2 (23.8%) (p < 0.05). CONCLUSION: IVM procedure is more efficient when it is performed before oocyte vitrification.