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Oestrogen-induced increase in uterine cGMP content in vitro: Effects of inhibitors of protein and RNA synthesis

par Flandroy, Lucette;Galand, Paul
Référence Molecular and cellular endocrinology, 25, 1, page (49-54)
Publication Publié, 1982

Article révisé par les pairs

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Résumé :

We have shown previously that oestradiol elevates the cGMP content of isolated uterine horns incubated for 2 h with the hormone. Cycloheximide (30 μg/ml) or actinomycin D (100 μg/ml), at concentrations which markedly inhibit protein and RNA synthesis, blocked the oestrogen-induced increase in cGMP. These agents do not inhibit the rise in uterine cGMP content provoked by sodium nitroprusside, thus arguing against a direct toxic effect on the enzyme guanylate cyclase. α-Amanitin, even at very high concentrations (80 μg/ml), interfered much less efficiently with total RNA and protein synthesis and also failed to prevent the oestrogen-induced increase in cGMP content. Taken together, these observations indicate that oestrogen action on uterine cGMP concentration in vitro depends on the RNA and/or a protein biosynthetic event that takes place in the uterus. This therefore confirms and extends analogous observations made previously under conditions in vivo.