par Azarkan, Mohamed ;Dibiani, Rachid ;Baulard, Céline ;Volant-Baeyens, Danielle
Référence International journal of biological macromolecules, 38, 3-5, page (216-224)
Publication Publié, 2006-05
Article révisé par les pairs
Résumé : The mechanical wounding impact on the Carica papaya latex protein pattern was investigated by analyzing three latexes. A first one commercially available, a second harvested from unripe but fully grown fruits, both obtained from regularly tapped fruits. A third one was collected from similar fruits but wounded for the first time. The results demonstrated both quantitative and qualitative changes in the protein content and in the enzymatic activity. Repeated wounding results in either, accumulation or activation (or both of them) of papain, chymopapain and caricain. Furthermore, new cysteine protease activity was found to transiently accumulate in the latex collected from newly wounded fruits. The possible implication of this enzymatic material in the papaya cysteine endopeptidases pro-forms activation is discussed.